Establishment of an efficient primary callus induction for embryogenic potential of Coffea arabica
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Aug 15, 2022
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Coffea arabica L. is a species of coffee that contribute for more than seventy percent of world coffee production. Various attempts have been made to obtain large quantities of planting material through propagation in vitro somatic embryogenesis technology. The objective of this experiment was to evaluate the effect of different plant growth regulators (PGRs) on callus induction (indirect somatic embryogenesis) in AS2K clone of Arabica coffee. Mother plants of Arabica coffee were established in coffee experimental field of Indonesian Coffee and Cocoa Research Institute at Andung Sari, Bondowoso, East Java, Indonesia (-7˚55'' ' S, 113˚41'' ' E) at an altitude of 1380,1 m dpl. Leaf explants were cultured on a half-strength Murashige and Skoog (MS) medium supplemented with various concentration (1.0, 2.0, 3.0 mg/L) of 2,4-D and (1.0, 2.0, 3.0 mg/L) thidiazuron in combination with 1.0 mg/L BAP. All the experiments were organized in completely random design (CDR) and repeated three times, each using minimum seven replicates (a total of 21 explants per treatment). The morphologycal and histological analysis of the different types of callus were observed. The percentage of callus formation was recorded every two weeks until eight weeks. The highest percentage of callus formation (almost 60%) was in medium containing 1 mg/L 2,4-D dan 1 mg/L BAP. Morphological and histological studies prove that the callus has a friable and embryogenic texture and begins to develop various stages of somatic embryo formation, starting with the globular, heart, torpedo and cotyledonary phases.
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Arimarsetiowati, R., Daryono, B., Astuti, Y., & Semiarti, E. (2022). Establishment of an efficient primary callus induction for embryogenic potential of Coffea arabica. Pelita Perkebunan (a Coffee and Cocoa Research Journal), 38(2), 108-119. https://doi.org/10.22302/iccri.jur.pelitaperkebunan.v38i2.510
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References
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Arimarsetiowati, R (2011). Pengaruh auksin 2,4-D dan sitokinin 2-ip terhadap pembentukan embriogenesis somatik langsung pada eksplan daun Coffea arabica L. Jurnal Pelita Perkebunan, 27(2), 68-76
Bartos, P.M.C., Gomes, H.T., Gomes, S.M. et al. (2018). Histology of somatic embryogenesis in Coffea arabica L.. Biologia,73, 1255–1265.
Bobadilla Landey, R., Cenci, A., Georget, F., Bertrand, B., Camayo, G., Dechamp, E.,Simpson, J., Herrera, J.C., Santoni, S., Lashermes, P., Etienne, H (2013). Highgenetic and epigenetic stability in Coffea arabica plants derived fromembryogenic suspensions and secondary embryogenesis as eevealed by AFLP,MSAP and the phenotypic variation rate. PLoS One, 8 (2) e56372
Furuta KM, Hellmann E, Helariutta Y (2014). Molecular control of cell specification and cell differentiation during procambial development. Annu Rev Plant Bio, 65, 607–638.
Irene WM, Alumiro HL2, Asava KK, Agwanda CO, Anami SE1 (2019). Effects of Genotype and Plant Growth Regulators on Callus Induction in Leaf Cultures of Coffea arabica L. F1 Hybrid. Journal of Plant Biochemistry & Physiology, 7(2), 236.
Kahia J, Kirika M, Lubabali H, Mantel S (2016). High-frequency direct somatic embryogenesis and plantlet regeneration from leaves derived from in vitro-germinated seedlings of a Coffea arabica hybrid cultivar. Hortscience, 51, 1148- 1152.
Kumar S, Singh R, Kalia S, Sharma SK, Kalia R (2016). Recent advances in understanding the role of growth regulators in plant growth and development in vitro-I Conventional growth regulators. Indian Forester, 142, 459-470.
Loyola-Vargas, V. M (2016). The history of somatic embryogenesis In V. M. Loyola-Vargas, & N. Ochoa-Alejo (Eds.), Somatic embryogenesis: fundamental aspects and applications. Switzerland: Springer International Publishing AG.
Mayerni, R., Satria, B., Wardhani, DK., Chan, SROS (2020). Effect of auxin (2,4-D) and cytokinin (BAP) in callus induction of local patchouli plants (Pogostemon cablin Benth.). IOP Conf. Ser.: Earth Environ. Sci. 583 012003.
Méndez-Hernández, H.A., Ledezma-Rodríguez, M., Avilez-Montalvo, R.N., Juarez-Gomez, Y.L., Skeete, A., Avilez-Montalvo, J., De-la-Peña, C., Loyola-Vargas Víctor, M (2019). Signaling overview of plant somatic embryogenesis. Front Plant Sci,10, 77.
Molina DM, Aponte ME, Cortina H et al. (2002) The effect of genotype and explant age on somatic embryogenesis of coffee. Plant Cell Tiss Org,71, 117–123.
Raji, M.R., Lotfi, M., Tohidfar, M., Zahedi, B., Carra, A., Abbate, L., Carimi, F (2018). Somatic embryogenesis of muskmelon (Cucumis melo L.) and genetic stability assessment of regenerants using flow cytometry and ISSR markers. Protoplasma, 255, 873–883.
Ribas AF, Dechamp E, Champion A, Bertrand B, Combes MC, Verdeil JL, Lapeyre F, Lashermes P, Etienne H (2011). Agrobacteriummediated genetic transformation of Coffea arabica (L.) is greatly enhanced by using established embryogenic callus cultures. BMC Plant Biol, 11, 92.
Rojas-Lorz L, Arrieta-Espinoza G, Valdez-Melara M, Pereira LFP, Gatica-Arias A (2019) Influence of silver nitrate on somatic embryogenesis induction in Arabica Coffee (Coffea arabica L.). Brazilian Archives of Biology and Technology, 62:e19180228.
Ruzin, SE (1999) Plant Microtechnique and Microscopy. Oxford University Press, New York Oxford pp. 322.
Sanglard, Natália Arruda; Amaral-Silva, Paulo Marcos; Sattler, Mariana Cansian; de Oliveira, Stéfanie Cristina; Cesário, Letícia Miranda; Ferreira, Adésio; Carvalho, Carlos Roberto; Clarindo, Wellington Ronildo (2019). Indirect somatic embryogenesis in Coffea with different ploidy levels: a revisiting and updating study. Plant Cell, Tissue and Organ Culture (PCTOC).
Silva, G. M., Cruz, A. C. F., Otoni, W. C., Pereira, T. N. S., Rocha, D. I., & Silva, M. L (2015). Histochemical evaluation of induction of somatic embryogenesis in Passiflora edulis Sims (Passifloraceae). In Vitro Cellular & Developmental Biology – Plant, 51, 539-545.
Singh, R., Kashyap, S.P., Kumari, N., Singh, M (2016). Regeneration of soapnut tree through somatic embryogenesis and assessment of genetic fidelity through ISSR and RAPD markers. Physiol. Mol. Biol. Plant, 22, 381–389.
Sujatha,M., Visarada, K (2013). Biolistic DNA delivery. In: Sudowe, S, Reske-Kunz, AB (Eds.), Transformation of Nuclear DNA in Meristematic and Embryogenic Tissues. Springer, NewYork, pp. 27–44.
Torres LF, Diniz LEC, do Livramento KG, Freire LL, Paiva LV (2015) Gene expression and morphological characterization of cell suspensions of Coffea arabica L. cv. Catiguá MG2 in different cultivation stages. Acta Physiol Plant, 37, 175.
Van Boxtel J, Berthouly M (1996). High frequency somatic embryogenesis from coffee leaves. Factors influencing embryogenesis, and subsequent proliferation and regeneration in liquid medium. Plant Cell Tiss Org, 44, 7–17.
Yi-Chieh W, Meng-Ze L, Bin H, Hsiao-Hang C, Jen-Tsung C (2018). Thidiazuron enhanced somatic embryogenesis from callus lines of arabica coffee and subsequent plant regeneration. Acta Biologica Cracoviensia Series Botanica, 60, 35-44.
Andrea Silva de Almeida, J (2020). Observations on Somatic Embryogenesis in Coffea arabica L. Coffee - Production and Research. Dalyse Toledo Castanheira, IntechOpen.
Ardiyani F (2015). Morphological characterization and identification of Coffea liberica callus of somatic embryogenesis propagation. Jurnal Pelita Perkebunan, 31, 81–89
Arimarsetiowati, R (2011). Pengaruh auksin 2,4-D dan sitokinin 2-ip terhadap pembentukan embriogenesis somatik langsung pada eksplan daun Coffea arabica L. Jurnal Pelita Perkebunan, 27(2), 68-76
Bartos, P.M.C., Gomes, H.T., Gomes, S.M. et al. (2018). Histology of somatic embryogenesis in Coffea arabica L.. Biologia,73, 1255–1265.
Bobadilla Landey, R., Cenci, A., Georget, F., Bertrand, B., Camayo, G., Dechamp, E.,Simpson, J., Herrera, J.C., Santoni, S., Lashermes, P., Etienne, H (2013). Highgenetic and epigenetic stability in Coffea arabica plants derived fromembryogenic suspensions and secondary embryogenesis as eevealed by AFLP,MSAP and the phenotypic variation rate. PLoS One, 8 (2) e56372
Furuta KM, Hellmann E, Helariutta Y (2014). Molecular control of cell specification and cell differentiation during procambial development. Annu Rev Plant Bio, 65, 607–638.
Irene WM, Alumiro HL2, Asava KK, Agwanda CO, Anami SE1 (2019). Effects of Genotype and Plant Growth Regulators on Callus Induction in Leaf Cultures of Coffea arabica L. F1 Hybrid. Journal of Plant Biochemistry & Physiology, 7(2), 236.
Kahia J, Kirika M, Lubabali H, Mantel S (2016). High-frequency direct somatic embryogenesis and plantlet regeneration from leaves derived from in vitro-germinated seedlings of a Coffea arabica hybrid cultivar. Hortscience, 51, 1148- 1152.
Kumar S, Singh R, Kalia S, Sharma SK, Kalia R (2016). Recent advances in understanding the role of growth regulators in plant growth and development in vitro-I Conventional growth regulators. Indian Forester, 142, 459-470.
Loyola-Vargas, V. M (2016). The history of somatic embryogenesis In V. M. Loyola-Vargas, & N. Ochoa-Alejo (Eds.), Somatic embryogenesis: fundamental aspects and applications. Switzerland: Springer International Publishing AG.
Mayerni, R., Satria, B., Wardhani, DK., Chan, SROS (2020). Effect of auxin (2,4-D) and cytokinin (BAP) in callus induction of local patchouli plants (Pogostemon cablin Benth.). IOP Conf. Ser.: Earth Environ. Sci. 583 012003.
Méndez-Hernández, H.A., Ledezma-Rodríguez, M., Avilez-Montalvo, R.N., Juarez-Gomez, Y.L., Skeete, A., Avilez-Montalvo, J., De-la-Peña, C., Loyola-Vargas Víctor, M (2019). Signaling overview of plant somatic embryogenesis. Front Plant Sci,10, 77.
Molina DM, Aponte ME, Cortina H et al. (2002) The effect of genotype and explant age on somatic embryogenesis of coffee. Plant Cell Tiss Org,71, 117–123.
Raji, M.R., Lotfi, M., Tohidfar, M., Zahedi, B., Carra, A., Abbate, L., Carimi, F (2018). Somatic embryogenesis of muskmelon (Cucumis melo L.) and genetic stability assessment of regenerants using flow cytometry and ISSR markers. Protoplasma, 255, 873–883.
Ribas AF, Dechamp E, Champion A, Bertrand B, Combes MC, Verdeil JL, Lapeyre F, Lashermes P, Etienne H (2011). Agrobacteriummediated genetic transformation of Coffea arabica (L.) is greatly enhanced by using established embryogenic callus cultures. BMC Plant Biol, 11, 92.
Rojas-Lorz L, Arrieta-Espinoza G, Valdez-Melara M, Pereira LFP, Gatica-Arias A (2019) Influence of silver nitrate on somatic embryogenesis induction in Arabica Coffee (Coffea arabica L.). Brazilian Archives of Biology and Technology, 62:e19180228.
Ruzin, SE (1999) Plant Microtechnique and Microscopy. Oxford University Press, New York Oxford pp. 322.
Sanglard, Natália Arruda; Amaral-Silva, Paulo Marcos; Sattler, Mariana Cansian; de Oliveira, Stéfanie Cristina; Cesário, Letícia Miranda; Ferreira, Adésio; Carvalho, Carlos Roberto; Clarindo, Wellington Ronildo (2019). Indirect somatic embryogenesis in Coffea with different ploidy levels: a revisiting and updating study. Plant Cell, Tissue and Organ Culture (PCTOC).
Silva, G. M., Cruz, A. C. F., Otoni, W. C., Pereira, T. N. S., Rocha, D. I., & Silva, M. L (2015). Histochemical evaluation of induction of somatic embryogenesis in Passiflora edulis Sims (Passifloraceae). In Vitro Cellular & Developmental Biology – Plant, 51, 539-545.
Singh, R., Kashyap, S.P., Kumari, N., Singh, M (2016). Regeneration of soapnut tree through somatic embryogenesis and assessment of genetic fidelity through ISSR and RAPD markers. Physiol. Mol. Biol. Plant, 22, 381–389.
Sujatha,M., Visarada, K (2013). Biolistic DNA delivery. In: Sudowe, S, Reske-Kunz, AB (Eds.), Transformation of Nuclear DNA in Meristematic and Embryogenic Tissues. Springer, NewYork, pp. 27–44.
Torres LF, Diniz LEC, do Livramento KG, Freire LL, Paiva LV (2015) Gene expression and morphological characterization of cell suspensions of Coffea arabica L. cv. Catiguá MG2 in different cultivation stages. Acta Physiol Plant, 37, 175.
Van Boxtel J, Berthouly M (1996). High frequency somatic embryogenesis from coffee leaves. Factors influencing embryogenesis, and subsequent proliferation and regeneration in liquid medium. Plant Cell Tiss Org, 44, 7–17.
Yi-Chieh W, Meng-Ze L, Bin H, Hsiao-Hang C, Jen-Tsung C (2018). Thidiazuron enhanced somatic embryogenesis from callus lines of arabica coffee and subsequent plant regeneration. Acta Biologica Cracoviensia Series Botanica, 60, 35-44.
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