Establishment of an efficient primary callus induction for embryogenic potential of Coffea arabica
Article Sidebar
PDF
VIEW
Published
Aug 15, 2022
DIMENSION
ALTMETRIC
Main Article Content
Abstract
Coffea arabica L. is a species of coffee that contribute for more than seventy percent of world coffee production. Various attempts have been made to obtain large quantities of planting material through propagation in vitro somatic embryogenesis technology. The objective of this experiment was to evaluate the effect of different plant growth regulators (PGRs) on callus induction (indirect somatic embryogenesis) in AS2K clone of Arabica coffee. Mother plants of Arabica coffee were established in coffee experimental field of Indonesian Coffee and Cocoa Research Institute at Andung Sari, Bondowoso, East Java, Indonesia (-7˚55'' ' S, 113˚41'' ' E) at an altitude of 1380,1 m dpl. Leaf explants were cultured on a half-strength Murashige and Skoog (MS) medium supplemented with various concentration (1.0, 2.0, 3.0 mg/L) of 2,4-D and (1.0, 2.0, 3.0 mg/L) thidiazuron in combination with 1.0 mg/L BAP. All the experiments were organized in completely random design (CDR) and repeated three times, each using minimum seven replicates (a total of 21 explants per treatment). The morphologycal and histological analysis of the different types of callus were observed. The percentage of callus formation was recorded every two weeks until eight weeks. The highest percentage of callus formation (almost 60%) was in medium containing 1 mg/L 2,4-D dan 1 mg/L BAP. Morphological and histological studies prove that the callus has a friable and embryogenic texture and begins to develop various stages of somatic embryo formation, starting with the globular, heart, torpedo and cotyledonary phases.
Article Details
How to Cite
Arimarsetiowati, R., Daryono, B., Astuti, Y., & Semiarti, E. (2022). Establishment of an efficient primary callus induction for embryogenic potential of Coffea arabica. Pelita Perkebunan (a Coffee and Cocoa Research Journal), 38(2), 108-119. https://doi.org/10.22302/iccri.jur.pelitaperkebunan.v38i2.510
Issue
Section
Articles
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
References
Almeida JAS, Silvarolla MB (2009). Induction of somatic embryos of Coffea arabica genotypes by 6-benzyladenine. International Journal of Plant Developmental Biology, 53, 5-8. Available from: http://www. globalsciencebooks.info/Online/ GSBOnline/OnlineIJPDB_3_1.html.
Andrea Silva de Almeida, J (2020). Observations on Somatic Embryogenesis in Coffea arabica L. Coffee - Production and Research. Dalyse Toledo Castanheira, IntechOpen.
Ardiyani F (2015). Morphological characterization and identification of Coffea liberica callus of somatic embryogenesis propagation. Jurnal Pelita Perkebunan, 31, 81–89
Arimarsetiowati, R (2011). Pengaruh auksin 2,4-D dan sitokinin 2-ip terhadap pembentukan embriogenesis somatik langsung pada eksplan daun Coffea arabica L. Jurnal Pelita Perkebunan, 27(2), 68-76
Bartos, P.M.C., Gomes, H.T., Gomes, S.M. et al. (2018). Histology of somatic embryogenesis in Coffea arabica L.. Biologia,73, 1255–1265.
Bobadilla Landey, R., Cenci, A., Georget, F., Bertrand, B., Camayo, G., Dechamp, E.,Simpson, J., Herrera, J.C., Santoni, S., Lashermes, P., Etienne, H (2013). Highgenetic and epigenetic stability in Coffea arabica plants derived fromembryogenic suspensions and secondary embryogenesis as eevealed by AFLP,MSAP and the phenotypic variation rate. PLoS One, 8 (2) e56372
Furuta KM, Hellmann E, Helariutta Y (2014). Molecular control of cell specification and cell differentiation during procambial development. Annu Rev Plant Bio, 65, 607–638.
Irene WM, Alumiro HL2, Asava KK, Agwanda CO, Anami SE1 (2019). Effects of Genotype and Plant Growth Regulators on Callus Induction in Leaf Cultures of Coffea arabica L. F1 Hybrid. Journal of Plant Biochemistry & Physiology, 7(2), 236.
Kahia J, Kirika M, Lubabali H, Mantel S (2016). High-frequency direct somatic embryogenesis and plantlet regeneration from leaves derived from in vitro-germinated seedlings of a Coffea arabica hybrid cultivar. Hortscience, 51, 1148- 1152.
Kumar S, Singh R, Kalia S, Sharma SK, Kalia R (2016). Recent advances in understanding the role of growth regulators in plant growth and development in vitro-I Conventional growth regulators. Indian Forester, 142, 459-470.
Loyola-Vargas, V. M (2016). The history of somatic embryogenesis In V. M. Loyola-Vargas, & N. Ochoa-Alejo (Eds.), Somatic embryogenesis: fundamental aspects and applications. Switzerland: Springer International Publishing AG.
Mayerni, R., Satria, B., Wardhani, DK., Chan, SROS (2020). Effect of auxin (2,4-D) and cytokinin (BAP) in callus induction of local patchouli plants (Pogostemon cablin Benth.). IOP Conf. Ser.: Earth Environ. Sci. 583 012003.
Méndez-Hernández, H.A., Ledezma-Rodríguez, M., Avilez-Montalvo, R.N., Juarez-Gomez, Y.L., Skeete, A., Avilez-Montalvo, J., De-la-Peña, C., Loyola-Vargas Víctor, M (2019). Signaling overview of plant somatic embryogenesis. Front Plant Sci,10, 77.
Molina DM, Aponte ME, Cortina H et al. (2002) The effect of genotype and explant age on somatic embryogenesis of coffee. Plant Cell Tiss Org,71, 117–123.
Raji, M.R., Lotfi, M., Tohidfar, M., Zahedi, B., Carra, A., Abbate, L., Carimi, F (2018). Somatic embryogenesis of muskmelon (Cucumis melo L.) and genetic stability assessment of regenerants using flow cytometry and ISSR markers. Protoplasma, 255, 873–883.
Ribas AF, Dechamp E, Champion A, Bertrand B, Combes MC, Verdeil JL, Lapeyre F, Lashermes P, Etienne H (2011). Agrobacteriummediated genetic transformation of Coffea arabica (L.) is greatly enhanced by using established embryogenic callus cultures. BMC Plant Biol, 11, 92.
Rojas-Lorz L, Arrieta-Espinoza G, Valdez-Melara M, Pereira LFP, Gatica-Arias A (2019) Influence of silver nitrate on somatic embryogenesis induction in Arabica Coffee (Coffea arabica L.). Brazilian Archives of Biology and Technology, 62:e19180228.
Ruzin, SE (1999) Plant Microtechnique and Microscopy. Oxford University Press, New York Oxford pp. 322.
Sanglard, Natália Arruda; Amaral-Silva, Paulo Marcos; Sattler, Mariana Cansian; de Oliveira, Stéfanie Cristina; Cesário, Letícia Miranda; Ferreira, Adésio; Carvalho, Carlos Roberto; Clarindo, Wellington Ronildo (2019). Indirect somatic embryogenesis in Coffea with different ploidy levels: a revisiting and updating study. Plant Cell, Tissue and Organ Culture (PCTOC).
Silva, G. M., Cruz, A. C. F., Otoni, W. C., Pereira, T. N. S., Rocha, D. I., & Silva, M. L (2015). Histochemical evaluation of induction of somatic embryogenesis in Passiflora edulis Sims (Passifloraceae). In Vitro Cellular & Developmental Biology – Plant, 51, 539-545.
Singh, R., Kashyap, S.P., Kumari, N., Singh, M (2016). Regeneration of soapnut tree through somatic embryogenesis and assessment of genetic fidelity through ISSR and RAPD markers. Physiol. Mol. Biol. Plant, 22, 381–389.
Sujatha,M., Visarada, K (2013). Biolistic DNA delivery. In: Sudowe, S, Reske-Kunz, AB (Eds.), Transformation of Nuclear DNA in Meristematic and Embryogenic Tissues. Springer, NewYork, pp. 27–44.
Torres LF, Diniz LEC, do Livramento KG, Freire LL, Paiva LV (2015) Gene expression and morphological characterization of cell suspensions of Coffea arabica L. cv. Catiguá MG2 in different cultivation stages. Acta Physiol Plant, 37, 175.
Van Boxtel J, Berthouly M (1996). High frequency somatic embryogenesis from coffee leaves. Factors influencing embryogenesis, and subsequent proliferation and regeneration in liquid medium. Plant Cell Tiss Org, 44, 7–17.
Yi-Chieh W, Meng-Ze L, Bin H, Hsiao-Hang C, Jen-Tsung C (2018). Thidiazuron enhanced somatic embryogenesis from callus lines of arabica coffee and subsequent plant regeneration. Acta Biologica Cracoviensia Series Botanica, 60, 35-44.
Andrea Silva de Almeida, J (2020). Observations on Somatic Embryogenesis in Coffea arabica L. Coffee - Production and Research. Dalyse Toledo Castanheira, IntechOpen.
Ardiyani F (2015). Morphological characterization and identification of Coffea liberica callus of somatic embryogenesis propagation. Jurnal Pelita Perkebunan, 31, 81–89
Arimarsetiowati, R (2011). Pengaruh auksin 2,4-D dan sitokinin 2-ip terhadap pembentukan embriogenesis somatik langsung pada eksplan daun Coffea arabica L. Jurnal Pelita Perkebunan, 27(2), 68-76
Bartos, P.M.C., Gomes, H.T., Gomes, S.M. et al. (2018). Histology of somatic embryogenesis in Coffea arabica L.. Biologia,73, 1255–1265.
Bobadilla Landey, R., Cenci, A., Georget, F., Bertrand, B., Camayo, G., Dechamp, E.,Simpson, J., Herrera, J.C., Santoni, S., Lashermes, P., Etienne, H (2013). Highgenetic and epigenetic stability in Coffea arabica plants derived fromembryogenic suspensions and secondary embryogenesis as eevealed by AFLP,MSAP and the phenotypic variation rate. PLoS One, 8 (2) e56372
Furuta KM, Hellmann E, Helariutta Y (2014). Molecular control of cell specification and cell differentiation during procambial development. Annu Rev Plant Bio, 65, 607–638.
Irene WM, Alumiro HL2, Asava KK, Agwanda CO, Anami SE1 (2019). Effects of Genotype and Plant Growth Regulators on Callus Induction in Leaf Cultures of Coffea arabica L. F1 Hybrid. Journal of Plant Biochemistry & Physiology, 7(2), 236.
Kahia J, Kirika M, Lubabali H, Mantel S (2016). High-frequency direct somatic embryogenesis and plantlet regeneration from leaves derived from in vitro-germinated seedlings of a Coffea arabica hybrid cultivar. Hortscience, 51, 1148- 1152.
Kumar S, Singh R, Kalia S, Sharma SK, Kalia R (2016). Recent advances in understanding the role of growth regulators in plant growth and development in vitro-I Conventional growth regulators. Indian Forester, 142, 459-470.
Loyola-Vargas, V. M (2016). The history of somatic embryogenesis In V. M. Loyola-Vargas, & N. Ochoa-Alejo (Eds.), Somatic embryogenesis: fundamental aspects and applications. Switzerland: Springer International Publishing AG.
Mayerni, R., Satria, B., Wardhani, DK., Chan, SROS (2020). Effect of auxin (2,4-D) and cytokinin (BAP) in callus induction of local patchouli plants (Pogostemon cablin Benth.). IOP Conf. Ser.: Earth Environ. Sci. 583 012003.
Méndez-Hernández, H.A., Ledezma-Rodríguez, M., Avilez-Montalvo, R.N., Juarez-Gomez, Y.L., Skeete, A., Avilez-Montalvo, J., De-la-Peña, C., Loyola-Vargas Víctor, M (2019). Signaling overview of plant somatic embryogenesis. Front Plant Sci,10, 77.
Molina DM, Aponte ME, Cortina H et al. (2002) The effect of genotype and explant age on somatic embryogenesis of coffee. Plant Cell Tiss Org,71, 117–123.
Raji, M.R., Lotfi, M., Tohidfar, M., Zahedi, B., Carra, A., Abbate, L., Carimi, F (2018). Somatic embryogenesis of muskmelon (Cucumis melo L.) and genetic stability assessment of regenerants using flow cytometry and ISSR markers. Protoplasma, 255, 873–883.
Ribas AF, Dechamp E, Champion A, Bertrand B, Combes MC, Verdeil JL, Lapeyre F, Lashermes P, Etienne H (2011). Agrobacteriummediated genetic transformation of Coffea arabica (L.) is greatly enhanced by using established embryogenic callus cultures. BMC Plant Biol, 11, 92.
Rojas-Lorz L, Arrieta-Espinoza G, Valdez-Melara M, Pereira LFP, Gatica-Arias A (2019) Influence of silver nitrate on somatic embryogenesis induction in Arabica Coffee (Coffea arabica L.). Brazilian Archives of Biology and Technology, 62:e19180228.
Ruzin, SE (1999) Plant Microtechnique and Microscopy. Oxford University Press, New York Oxford pp. 322.
Sanglard, Natália Arruda; Amaral-Silva, Paulo Marcos; Sattler, Mariana Cansian; de Oliveira, Stéfanie Cristina; Cesário, Letícia Miranda; Ferreira, Adésio; Carvalho, Carlos Roberto; Clarindo, Wellington Ronildo (2019). Indirect somatic embryogenesis in Coffea with different ploidy levels: a revisiting and updating study. Plant Cell, Tissue and Organ Culture (PCTOC).
Silva, G. M., Cruz, A. C. F., Otoni, W. C., Pereira, T. N. S., Rocha, D. I., & Silva, M. L (2015). Histochemical evaluation of induction of somatic embryogenesis in Passiflora edulis Sims (Passifloraceae). In Vitro Cellular & Developmental Biology – Plant, 51, 539-545.
Singh, R., Kashyap, S.P., Kumari, N., Singh, M (2016). Regeneration of soapnut tree through somatic embryogenesis and assessment of genetic fidelity through ISSR and RAPD markers. Physiol. Mol. Biol. Plant, 22, 381–389.
Sujatha,M., Visarada, K (2013). Biolistic DNA delivery. In: Sudowe, S, Reske-Kunz, AB (Eds.), Transformation of Nuclear DNA in Meristematic and Embryogenic Tissues. Springer, NewYork, pp. 27–44.
Torres LF, Diniz LEC, do Livramento KG, Freire LL, Paiva LV (2015) Gene expression and morphological characterization of cell suspensions of Coffea arabica L. cv. Catiguá MG2 in different cultivation stages. Acta Physiol Plant, 37, 175.
Van Boxtel J, Berthouly M (1996). High frequency somatic embryogenesis from coffee leaves. Factors influencing embryogenesis, and subsequent proliferation and regeneration in liquid medium. Plant Cell Tiss Org, 44, 7–17.
Yi-Chieh W, Meng-Ze L, Bin H, Hsiao-Hang C, Jen-Tsung C (2018). Thidiazuron enhanced somatic embryogenesis from callus lines of arabica coffee and subsequent plant regeneration. Acta Biologica Cracoviensia Series Botanica, 60, 35-44.
Most read articles by the same author(s)
- Rina Arimarsetiowati, Fitria Ardiyani, Pengaruh penambahan auxin terhadap pertunasan dan perakaran kopi arabika perbanyakan Somatic Embryogenesis (The effects of shooting and rooting of arabica coffee propagation through Embryogenesis Somatic auxin uses). , Pelita Perkebunan (a Coffee and Cocoa Research Journal): Vol 28 No 2 (2012)
- Rina Arimarsetiowati, Embryo Germination Development of Coffea arabica L.at Various Media Composition, Subcultures Stages and Embryo Size , Pelita Perkebunan (a Coffee and Cocoa Research Journal): Vol 33 No 3 (2017)
- Erwin Prastowo, Novie Pranata Erdiansyah, Rina Arimarsetiowati, Leaf Mineral Composition of Coffee Infected by a Hemileia vastatrix Fungus in Bondowoso, East Java , Pelita Perkebunan (a Coffee and Cocoa Research Journal): Vol 35 No 1 (2019)
- Erwin Prastowo, Rina Arimarsetiowati, Characteristics of coffee as a response to different environmental conditions in Lampung: I. Morphological variations , Pelita Perkebunan (a Coffee and Cocoa Research Journal): Vol 35 No 2 (2019)
- Erwin Prastowo, Rina Arimarsetiowati, Morphological Variations of Robusta Coffee As a Response to Different Altitude in Lampung , Pelita Perkebunan (a Coffee and Cocoa Research Journal): Vol 35 No 2 (2019)
- Rina Arimarsetiowati, Endang Semiarti, Budi Setiadi Daryono, Yohana Theresia Maria Astuti, Erwin Prastowo, In silico study of RKD4 gene function in Coffea arabica L. and various cultivated plants related to embryo formation initiation , Pelita Perkebunan (a Coffee and Cocoa Research Journal): Vol 40 No 2 (2024)