Main Article Content
Breeding for cocoa pod borer (CPB) resistance on cocoa was initiated by selecting the resistant genotypes through cocoa farm in the endemic area. For breeding purpose the collected genotypes should be assessed for their diversity in constructing appropriate mating design. This research has objective to assess genetic diversity of the exploratory collection using DNA fingerprinting. The tested clones were 25 exploratory collections compared to the references of seven international clones from International Cocoa Genebanks, Trinidad and12 national clones. DNA of the tested clones was sequenced using 15 SSR markers at Sustainable Perennial Crop Laboratory of Beltsville Agriculture Research Center.Data of fingerprinting were analyzed using GenAlEx program to perform number of alleles, observed heterozygosity (Ho), expected heterozygosity (He) and genetic distance. The tested clones performed high genetic diversity with the number of alleles was 98 (6.53 per locus) compared to the national clones andinternational clones were 95 (6.33 per locus) and 86 (5.73 per locus), respectively. The observed heterozygosity of the tested clones (Ho = 0.63) was higher thannational clones (Ho = 0.60) and international clones (Ho = 0.49) then supported by the expected heterozygosity (He) of the tested clones (He = 0.71) also highin equal value with international clones (He = 0.74). Cluster analysis grouped the tested clones to three main genetic groups, namely Trinitario, Forastero and unidentified group. The CPB-resistant clones of ARDACIAR 10 and KW 397 were clustered in different group that indicate a far distance of their genetic background.Key words:Genetic diversity, cocoa, CPB resistance, simple sequence repeat marker.
How to Cite
Wahyu Susilo, A., Zhang, D., & Motilal, L. (2013). Assessing Genetic Diversity Cocoa (Theobroma cacaoL.) Collection Resistant to Cocoa Pod Borer Using Simple Sequence Repeat Markers. Pelita Perkebunan (a Coffee and Cocoa Research Journal), 29(1). https://doi.org/10.22302/iccri.jur.pelitaperkebunan.v29i1.186
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).