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Most reliable and efficient protocol for Coffea arabica L. of Sigararutang variety plant regeneration was established using embryoid as an early explant from the induction of embryogenic callus phase. A completely randomized designs with 5 replications was designed to accomplish 20 protocols of embryo germina-tion methods with different steps of subculture, size of embryo and germination medium. The embryogenic calluses from the flush leave explant were induced embryoid on a half-strength MS medium fortified with a half-strength combina-tion vitamin of 1.8 mg/L nicotinic acid, 10.1 mg/L thiamin HCl and 3.1 mg/L pyri-doxine, 50 mg/L myo inositol, 33 mg/L L.cistein, 1 mg/L Kinetin, 0.1 mg/L NAA, 20 gr/L sucrose, 2.4 gr/L gelrite and pH 5.5.The result showed that Protocol 17 was the most effective, with 59,2% of rooted cotyledons, 4.04 cm of length of roots, 1.68 cm of length of hypocotyl, 20.8% of opened cotyledons and 100% of cotyledonary embryo at the end of 8 weeks which used the B medium, large embryos and twice phase of subculture from liquid medium to solid medium. The Protocol 17 is stable protocol from low to high value. Protocol 8 is the steady protocol from high to low value. Protocols 17 and 8 are the highest and lowest ranking, respectively, for each parameter. Protocol 17 is the most suitable for the germination embryo somatic.
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